glioblastoma homeopathic treatment

as in the case of glioma cells, rue stimulates the activation of erk1/2 and akt in a1 cells, whereas their blockade by pharmacological inhibitors prevents cell death. thus, a number of selective inhibitors of molecules and/or pathways involved in the pathogenesis of glioblastoma have been developed and some of them entered clinical trials. the present study was aimed to assess the effects of the aqueous extract of r. graveolens on the proliferation of human glioma cells and of neural progenitors from mouse cns, in comparison to differentiated, non-proliferating neural cells. mtt assay: cells were seeded at 3×105 cells/well in a 24 well plate in the presence or absence of 1mg/ml of r graveolens a.e., and cell proliferation assessed after 24, 48 and 72 hours. briefly, cells were seeded at 106 cells/plate in 60mm plates, treated or not with 1mg/ml of r. graveolens a.e. to assess the antiproliferative effects of r. graveolens water extract, mtt reduction assay and trypan blue exclusion test were performed on the human glioblastoma cell line u87mg. 2b, the treatment of a1 cells with 1 mg/ml r. graveolens a.e. promotes cell death in glioma and a1 cells, we analyzed the modulation of erk1/2 and akt cascades by means of western blotting.




these experiments revealed that also the inhibition of r. graveolens-activated akt rescued cell viability impairment as compared to cells treated with r. graveolens a.e. noteworthy, no changes in the number of necrotic cells was observed in treated and control cells (fig. interestingly, upon differentiation and cell cycle exit, a1 cells become insensitive to the noxious effects of r. graveolens extract. importantly, in our model these drugs, differently form r. graveolens extract, were not able to discriminate among glioma or proliferating a1 cells and differentiated a1 cells causing a significant cell death in all the cell populations analysed. however, a similar paradoxical activation of both erk1/2 and akt was reported in glioma cell lines to mediate the antiproliferative activity of adiponectin [78], confirming that in glioma cells over-activation of these kinase cascades is responsible of antiproliferative effects. mtt assay on proliferating c6 glioma cells (a) and u138 human glioma cells (b) treated with vehicle (♦) or with 1mg/ml r. graveolens a.e. western blotting detection of p-erk1/2 and erk1/2 proteins in c6 glioma cells (a), in u138 glioma cells (b), in a1 cells (c) and in u87mg (d) treated with 1mg/ml r. graveolens a.e. (a) western blotting detection of p-akt and akt proteins in u87mg (a), in c6 glioma cells (b) and in a1 cells (c) treated with 1mg/ml r. graveolens a.e. trypan blue exclusion test on proliferating a1 cells treated with or without (control) increasing concentrations of rutin (3μg/ml, 30μg/ml and 300μg/ml) for 24, 48 and 72h.

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it is therefore necessary to find alternative agents that can kill cancer cells but have minimal effects on normal cells. we investigated the brain cancer cell-killing activity of a homeopathic medicine, ruta, isolated from a plant, ruta graveolens. normal human blood lymphocytes, b-lymphoid cells, and brain cancer cells treated with ruta in vitro were examined for telomere dynamics, mitotic catastrophe, and apoptosis to understand the possible mechanism of cell-killing, using conventional and molecular cytogenetic techniques. we propose that ruta in combination with ca3(po4)2 could be used for effective treatment of brain cancers, particularly glioma.

r. graveolens aqueous extract induces cell death of glioma cell lines and of proliferating mesencephalic cells. to assess the antiproliferative effects of r. we investigated the brain cancer cell-killing activity of a homeopathic medicine, ruta, isolated from a plant, ruta graveolens. we treated human brain chemotherapy and radiation therapy are typically the recommended course of treatment for nearly all glioblastomas. following surgery, carmustine, related conditions, related conditions, healed from glioblastoma, best glioblastoma treatment in the world, glioblastoma new treatment 2020.

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